Effect of Cassiopea andromeda Venom on P15INK4b, P21 WAF1/CIP1, P53, DNA methyltransferase 1, and Bcl-2 Genes Expression, Apoptosis Induction, and Cell Growth Inhibition in Acute Promyelocytic Leukemia NB4 Cell line

Background: One of the acute hematologic malignancies is promyelocytic leukemia (APL) that resulted in translocation chromosomes 15 and 17, t (15; 17), cessation maturation myeloid cell line, ultimate aggregation neoplastic promyelocytes. Regarding appetence using herbal marine medicine studies increasing, on other hand, features Cassiopea andromeda Venom remained unclear; this study was conducted to determine its effects NB4 cells as a model for APL.
 Materials Methods: In experimental study, were treated with C. concentrations at different periods times. Growth inhibition toxic evaluated through methyl thiazole tetrazolium salt reduction (MTT test). The flow cytometry analysis carried out 7AAD Annexin V stains evaluating venom’s effect apoptotic pathways. Besides, Real-Time polymerase chain reaction performed evaluate relative gene expression.
 Results: inhibited growth correlated concentration time. Cell by 49.1%, after 24 hours treating 1000µg/mL Venom. This venom increased process, which then verified 7AAD/AnnexinV staining. fold change p15INK4b, p21 WAF1/CIP1, P53, DNMT1, Bcl-2 genes line 144, 2.78, 1.75, 15.24, 0.33, respectively, meant expression level DNMT1 14400%, 278%, 175%, 1524%, respectively decreased 67%.
 Conclusion: Considering inhibitory property Venom, authors recommended it part combinational medication APL animal trials leukemias’ vitro studies.